Beta-Actin Housekeeping Gene: A Reliable Marker for Human DNA Detection in Blood and Serum

In molecular diagnostics, maintaining the quality and integrity of collected specimens is crucial for obtaining reliable results. While external controls, such as a bacteriophage, validate the nucleic acid extraction process, they do not assess specimen integrity or the effectiveness of the collection method itself. This gap in control introduces potential risks, especially for self-collected or low-yield samples like serum.

Beta-Actin, a well-established housekeeping gene, is known for its consistent detection and expression across a variety of sample types and has been widely used in molecular assays. This study explores the potential of Beta-Actin as an internal control to verify specimen integrity and ensure effective DNA extraction. By integrating Beta-Actin into our clinical workflows, we aim to introduce an additional layer of control that will enhance the reliability and adaptability of our assays.

06 Mar 2025